Neoschizomer
Neoschizomers are restriction enzymes that recognize the same nucleotide sequence, but cleave at different sites. The first restriction enzyme discovered to recognize a sequence is called the prototype, and others that recognize the same sequence are isoschizomers. Neoschizomers are a subset of isoschizomers.[1]
For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓.[2]
Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG).[3]
Use in molecular biology
External links
- Rebase restriction enzyme database, http://rebase.neb.com/rebase/
See also
Wikidata has the property:
neoschizomer (P4875) (see uses)
References
- ^ "Isoschizomers". New England Biolabs. Retrieved 2024-09-12.
- ^ Welch, S. (1996-05-15). "Tsp49I (ACGT↓), a Thermostable Neoschizomer of the Type II Restriction Endonuclease Mae II (A↓CGT), Discovered in Isolates of the Genus Thermus from the Azores, Iceland and New Zealand". Nucleic Acids Research. 24 (10): 1799–1801. doi:10.1093/nar/24.10.1799. PMC 145888. PMID 8657557.
- ^ Withers, Barbara E.; Dunbar, Joan C. (1995). "DNA determinants in sequence-specific recognition by Xm al endonuclease". Nucleic Acids Research. 23 (17): 3571–3577. doi:10.1093/nar/23.17.3571. PMC 307239. PMID 7567471.