Reverse transcriptase

RNA-directed DNA polymerase
RNA-directed DNA polymerase
Identifiers
EC number	2.7.7.49
CAS number	9068-38-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
PMC
Search
PMC	articles
PubMed	articles
NCBI	proteins

Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe; PDBj
PDBsum	structure summary

Reverse transcriptase
(RNA-dependent DNA polymerase)
Reverse transcriptase; (RNA-dependent DNA polymerase)
	Crystallographic structure of HIV-1 reverse transcriptase where the two subunits p51 and p66 are colored and the active sites of polymerase and nuclease are highlighted.
Identifiers
Symbol	RVT_1
Pfam	PF00078
Pfam clan	CL0027
InterPro	IPR000477
PROSITE	PS50878
SCOP	1hmv
SUPERFAMILY	1hmv
CDD	cd00304
Pfam
Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe; PDBj
PDBsum	structure summary

Reverse transcriptase (RT) යනු RNA template එකකින් reverse transcription ක්‍රමවේදය හරහා complementary DNA (cDNA) නිපදවීමේදී භාවිතාවන එන්සයිමයක් වේ.

ඉතිහාසය

University of Wisconsin–Madison හි Howard Temin විසින් reverse transcriptases සොයාගත් අතර ^[2] 1970 දී MIT හි David Baltimore සිදුකරන ලද ස්වාදීන පර්යේෂණයක දී RNA tumour වෛරස දෙකක් වන murine leukemia virus සහ Rous sarcoma virus වලින් reverse transcriptase වෙන් කර ගන්නා ලදී.^[3] එම සොයාගැනීමට 1975 දී ඔවුන් දෙදෙනා හට Nobel Prize in Physiology or Medicine (Renato Dulbecco සමඟ) හීමිවිය.

ආශ්‍රිත

cDNA library
DNA polymerase
msDNA
Reverse transcribing virus
RNA polymerase
Telomerase
Retrotransposon marker

මූලාශ්‍ර

^ PDB: 3KLF; Tu X, Das K, Han Q, Bauman JD, Clark AD, Hou X, Frenkel YV, Gaffney BL, Jones RA, Boyer PL, Hughes SH, Sarafianos SG, Arnold E (October 2010). "Structural basis of HIV-1 resistance to AZT by excision". Nature Structural & Molecular Biology. 17 (10): 1202–9. doi:10.1038/nsmb.1908. PMC 2987654. PMID 20852643.
^ Temin HM, Mizutani S (June 1970). "RNA-dependent DNA polymerase in virions of Rous sarcoma virus". Nature. 226 (5252): 1211–3. doi:10.1038/2261211a0. PMID 4316301.
^ Baltimore D (June 1970). "RNA-dependent DNA polymerase in virions of RNA tumour viruses". Nature. 226 (5252): 1209–11. doi:10.1038/2261209a0. PMID 4316300.

භාහිර සබැඳි

RNA+Transcriptase at the US National Library of Medicine Medical Subject Headings (MeSH)
animation of reverse transcriptase action and three reverse transcriptase inhibitors
Molecule of the month සංරක්ෂණය කළ පිටපත 2020-04-09 at the Wayback Machine (September 2002) at the RCSB PDB
HIV Replication 3D Medical Animation. (Nov 2008). Video by Boehringer Ingelheim.
Goodsell DS. "Molecule of the Month: Reverse Transcriptase (Sep 2002)". Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB). සම්ප්‍රවේශය 2013-01-13.
PDBe-KB provides an overview of all the structure information available in the PDB for Human immunodeficiency virus Reverse transcriptase

[pmid20852643-1] PDB: 3KLF; Tu X, Das K, Han Q, Bauman JD, Clark AD, Hou X, Frenkel YV, Gaffney BL, Jones RA, Boyer PL, Hughes SH, Sarafianos SG, Arnold E (October 2010). "Structural basis of HIV-1 resistance to AZT by excision". Nature Structural & Molecular Biology. 17 (10): 1202–9. doi:10.1038/nsmb.1908. PMC 2987654. PMID 20852643.

[pmid4316301-2] Temin HM, Mizutani S (June 1970). "RNA-dependent DNA polymerase in virions of Rous sarcoma virus". Nature. 226 (5252): 1211–3. doi:10.1038/2261211a0. PMID 4316301.

[pmid4316300-3] Baltimore D (June 1970). "RNA-dependent DNA polymerase in virions of RNA tumour viruses". Nature. 226 (5252): 1209–11. doi:10.1038/2261209a0. PMID 4316300.

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